Anti-SCLT1 Antibody Picoband™

Product Name

Anti-SCLT1 Antibody Picoband™

View all Sclt1 Antibodies

SKU/Catalog Number

A11417-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SCLT1 Antibody Picoband™ catalog # A10225-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SCLT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11417-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human SCLT1 recombinant protein (Position: F19-I688). Human SCLT1 shares 76.7% amino acid (aa) sequence identity with both mouse and rat SCLT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A11417-1 is reactive to Sclt1 in Human

Applications

A11417-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

80.492kDa

Background of Sclt1

This gene encodes an adaptor protein. Studies of a related gene in rat suggest that the encoded protein functions to link clathrin to the sodium channel protein type 10 subunit alpha protein. The encoded protein has also been identified as a component of distal appendages of centrioles that is necessary for ciliogenesis. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SCLT1 using anti-SCLT1 antibody (A11417-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCLT1 antigen affinity purified polyclonal antibody (Catalog # A11417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCLT1 at approximately 75 kDa. The expected band size for SCLT1 is at 81 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-SCLT1 antibody (A11417-1).
Overlay histogram showing 293T cells stained with A11417-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCLT1 Antibody (A11417-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Sclt1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ36042; MGC70542; sodium channel and clathrin linker 1 Sclt1|2610207F23Rik, 4931421F20Rik, CAGGS-S, CAGGS-SB10, Tg(ACTB-sb10)1Dla, Tg(CAG-sb10)1Dla|sodium channel and clathrin linker 1|sodium channel and clathrin linker 1|sodium channel-associated protein 1

For more info on Sclt1, check out the Sclt1 Infographic

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In this infographic, you will see the following information for Sclt1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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